Abstract
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.
Original language | English |
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Pages (from-to) | 2540-5 |
Number of pages | 6 |
Journal | Genes & Development |
Volume | 19 |
Issue number | 21 |
DOIs | |
Publication status | Published - 1 Nov 2005 |
Keywords
- chromatin remodeling
- ecdysteroid
- ISWI
- chromatin
- NURF
- Drosophila