Projects per year
Abstract
The aquatic system is a major sink for engineered nanomaterials released into the environment. Here, we assessed the toxicity of graphene oxide (GO) using the freshwater planarian Dugesia japonica, an invertebrate model that has been widely used for studying the effects of toxins on tissue regeneration and neuronal development. GO not only impaired the growth of normal (homeostatic) worms, but also inhibited the regeneration processes of regenerating (amputated) worms, with LC10 values of 9.86 mg/L and 9.32 mg/L for the 48-h acute toxicity test, respectively. High concentration (200 mg/L) of GO killed all the worms after 3 (regenerating) or 4 (homeostasis) days of exposure. Whole-mount in situ hybridization (WISH) and immunofluorescence analyses suggest GO impaired stem cell proliferation and differentiation, and subsequently caused cell apoptosis and oxidative DNA damage during planarian regeneration. Mechanistic analysis suggests that GO disturbed the antioxidative system (enzymatic and non-enzymatic) and energy metabolism in the planarian at both molecular and genetic levels, thus causing reactive oxygen species (ROS) over accumulation and oxidative damage, including oxidative DNA damage, loss of mitochondrial membrane integrity, lack of energy supply for cell differentiation and proliferation leading to retardance of neuron regeneration. The intrinsic oxidative potential of GO contributes to the GO-induced toxicity in planarians. These data suggest that GO in aquatic systems can cause oxidative stress and neurotoxicity in planarians. Overall, regenerated tissues are more sensitive to GO toxicity than homeostatic ones, suggesting that careful handling and appropriate decisions are needed in the application of GO to achieve healing and tissue regeneration.
Original language | English |
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Article number | 114431 |
Number of pages | 10 |
Journal | Ecotoxicology and Environmental Safety |
Volume | 249 |
Early online date | 13 Dec 2022 |
DOIs | |
Publication status | Published - 1 Jan 2023 |
Bibliographical note
Funding Information:This work was supported by the National Natural Science Foundation of China (Grant Nos. 12105163 , 32001987 ) and the Natural Science Foundation of Shandong Province (Grant Nos. ZR2020QD133 , ZR2020QC241 , ZR2020MC142 ). Additional support from H2020 project NanoCommons (Grant Agreement No. 731032 ) and NanoSolveIT (Grant Agreement 814572 ) are acknowledged. Funding supports from the Royal Society International Exchange Programs ( 1853690 and 2122860 ) are also acknowledged.
Publisher Copyright:
© 2022
Keywords
- Development toxicity
- Graphene oxide
- Oxidative stress
- Planarian
- Regeneration
- Stem cell
ASJC Scopus subject areas
- Pollution
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis
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Modelling and prediction of the uptake and accumulation of nanomaterials in the soil-plant system
Zhang, P.
1/11/22 → 31/10/24
Project: Research Councils
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Nano-enabled Strategies to Enhance Plant Tolerance to Environmental Stress
Zhang, P.
31/03/22 → 30/03/24
Project: Research Councils
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H2020_RIA_NANOCOMMONS_CO-ORDINATOR
Valsami-Jones, E., Lynch, I. & Gkoutos, G.
1/01/18 → 30/06/22
Project: EU