Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

James P. C. Coverdale; Hugo A. van den Berg; Siavash Khazaipoul; Hannah E. Bridgewater; Alan J. Stewart; Claudia A. Blindauer

doi:10.1039/D2CC02278H

Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

James P. C. Coverdale, Hugo A. van den Berg, Siavash Khazaipoul, Hannah E. Bridgewater, Alan J. Stewart, Claudia A. Blindauer^*

^*Corresponding author for this work

Pharmacy

Research output: Contribution to journal › Article › peer-review

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Abstract

The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically-defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

Original language	English
Pages (from-to)	7384-7387
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	53
Early online date	2 Jun 2022
DOIs	https://doi.org/10.1039/D2CC02278H
Publication status	Published - 7 Jul 2022

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CoverdaleJ2022AlbuminFinal published version, 2.05 MBLicence: Creative Commons: Attribution (CC BY)

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title = "Albumin-mediated extracellular zinc speciation drives cellular zinc uptake",

abstract = "The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically-defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.",

author = "Coverdale, {James P. C.} and Berg, {Hugo A. van den} and Siavash Khazaipoul and Bridgewater, {Hannah E.} and Stewart, {Alan J.} and Blindauer, {Claudia A.}",

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AU - Coverdale, James P. C.

AU - Berg, Hugo A. van den

AU - Khazaipoul, Siavash

AU - Bridgewater, Hannah E.

AU - Stewart, Alan J.

AU - Blindauer, Claudia A.

PY - 2022/7/7

Y1 - 2022/7/7

N2 - The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically-defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

AB - The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically-defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

U2 - 10.1039/D2CC02278H

DO - 10.1039/D2CC02278H

M3 - Article

SN - 1359-7345

VL - 58

SP - 7384

EP - 7387

JO - Chemical Communications

JF - Chemical Communications

IS - 53

ER -

Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

Abstract

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