Projects per year
Abstract
BACKGROUND: Cardiac optical mapping enables direct and high spatio-temporal resolution recording of action potential (AP) morphology. Temporal alterations in AP morphology are both predictive and consequent of arrhythmia. Here we sought to test if methods that quantify regularity of recorded waveforms could be applied to detect and quantify periods of temporal instability in optical mapping datasets in a semi-automated, user-unbiased manner.
METHODS AND RESULTS: We developed, tested and applied algorithms to quantify optical wave similarity (OWS) to study morphological temporal similarity of optically recorded APs. Unlike other measures (e.g. alternans ratio, beat-to-beat variability, arrhythmia scoring), the quantification of OWS is achieved without a restrictive definition of specific signal points/features and is instead derived by analysing the complete morphology from the entire AP waveform. Using model datasets, we validated the ability of OWS to measure changes in AP morphology, and tested OWS mapping in guinea pig hearts and mouse atria. OWS successfully detected and measured alterations in temporal regularity in response to several proarrhythmic stimuli, including alterations in pacing frequency, premature contractions, alternans and ventricular fibrillation.
CONCLUSION: OWS mapping provides an effective measure of temporal regularity that can be applied to optical datasets to detect and quantify temporal alterations in action potential morphology. This methodology provides a new metric for arrhythmia inducibility and scoring in optical mapping datasets.
Original language | English |
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Journal | Progress in Biophysics and Molecular Biology |
Early online date | 30 Dec 2019 |
DOIs | |
Publication status | E-pub ahead of print - 30 Dec 2019 |
Keywords
- Optical mapping
- Wave similarity
- Regularity index
- Temporal stability
- Optical action potential
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Dive into the research topics of 'Temporal irregularity quantification and mapping of optical action potentials using wave morphology similarity'. Together they form a unique fingerprint.Projects
- 4 Finished
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Investigating regulatory pathways for intracellular sodium in the atria
1/10/19 → 30/04/23
Project: Research
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Scarred by kidney disease: FGF23 as a key mediator of cardiac fibrosis in patients with chronic kidney disease
Weston, C., Pavlovic, D., McGettrick, H. & Gough, R.
1/08/19 → 28/02/23
Project: Research
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Autonomic Modulation of Torsades de Pointes in Acquired Long QT Syndrome
Winter, J.
1/01/18 → 30/06/19
Project: Research