Abstract
Introduction: This study aimed to characterize the effectiveness of dentin-conditioning with bio-mineralizable chitosan-hydroxyapatite precursor (CS-HA) nanocomplexes alone or associated with tricalcium silicate sealer (TCS/CS-HA) on the mechanical property and antibiofilm efficacy in root dentin.
Methods: Flow tests were performed following ISO6876:2012-specifications. Solubility was measured. Micromorphology was assessed using Scanning Electron Microscopy (SEM). Nanohardness/elastic modulus were also determined. Fracture resistance was determined on lower premolars that were prepared, and randomly distributed among 7-groups (n=8/group), including the control, CS-HA dentin-conditioning and root canal filled groups. Similar canal preparation/distribution procedure was followed to test the antibacterial effect on Enterococcus faecalis-infected roots. Descriptive statistic was used to report SEM findings. Flowability results were analyzed using Paired t-test. Multiple comparisons from solubility, fracture and antibacterial assays were assessed by one-way ANOVA-Tukey’s tests.
Results: TCS/CS-HA showed optimal flow and no effect on solubility after immersion for 4 weeks (p>.05). TCS/CS-HA significantly increased nanohardness and elastic modulus (210±11.3MPa, 7.9±0.9GPa) compared to TCS (44.5±7.8MPa, 2.1±0.3GPa,p<.05). SEM revealed needle-shaped mineralized structures resulting in fewer voids and a well-adapted sealer-dentin interface in the TCS/CS-HA groups. NaOCl-EDTA irrigation resulted in reduced fracture resistance (444.34N) while CS-HA dentin-conditioning alone (928.28N, p<.05) and CS-HA dentin-conditioning plus CS-HA/TCS resulted in higher fracture resistance (1134.06N, p<.05). CS-HA dentin-conditioning also reduced bacteria by 2.04 log (4.50±0.43) from the initial bacterial load (6.54±0.07, p<.05). There was further bacterial reduction when CS-HA-conditioned root canals were filled with TCS or TCS/CS-HA (0.00 to 0.98±0.57, p>.05).
Conclusion: Dentin modification with CS-HA increased the fracture resistance of root dentin, and decreased the residual bacterial burden. TCS/CS-HA potentiated the nanomechanical and physical properties of TCS.
Methods: Flow tests were performed following ISO6876:2012-specifications. Solubility was measured. Micromorphology was assessed using Scanning Electron Microscopy (SEM). Nanohardness/elastic modulus were also determined. Fracture resistance was determined on lower premolars that were prepared, and randomly distributed among 7-groups (n=8/group), including the control, CS-HA dentin-conditioning and root canal filled groups. Similar canal preparation/distribution procedure was followed to test the antibacterial effect on Enterococcus faecalis-infected roots. Descriptive statistic was used to report SEM findings. Flowability results were analyzed using Paired t-test. Multiple comparisons from solubility, fracture and antibacterial assays were assessed by one-way ANOVA-Tukey’s tests.
Results: TCS/CS-HA showed optimal flow and no effect on solubility after immersion for 4 weeks (p>.05). TCS/CS-HA significantly increased nanohardness and elastic modulus (210±11.3MPa, 7.9±0.9GPa) compared to TCS (44.5±7.8MPa, 2.1±0.3GPa,p<.05). SEM revealed needle-shaped mineralized structures resulting in fewer voids and a well-adapted sealer-dentin interface in the TCS/CS-HA groups. NaOCl-EDTA irrigation resulted in reduced fracture resistance (444.34N) while CS-HA dentin-conditioning alone (928.28N, p<.05) and CS-HA dentin-conditioning plus CS-HA/TCS resulted in higher fracture resistance (1134.06N, p<.05). CS-HA dentin-conditioning also reduced bacteria by 2.04 log (4.50±0.43) from the initial bacterial load (6.54±0.07, p<.05). There was further bacterial reduction when CS-HA-conditioned root canals were filled with TCS or TCS/CS-HA (0.00 to 0.98±0.57, p>.05).
Conclusion: Dentin modification with CS-HA increased the fracture resistance of root dentin, and decreased the residual bacterial burden. TCS/CS-HA potentiated the nanomechanical and physical properties of TCS.
| Original language | English |
|---|---|
| Journal | Journal of Endodontics |
| Early online date | 7 Jul 2022 |
| DOIs | |
| Publication status | E-pub ahead of print - 7 Jul 2022 |
Keywords
- Chitosan
- dentin
- hydroxyapatite
- nanocomplexes
- tricalcium silicate sealer