QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY

Yujie Zheng; Samantha J. Montague; Yean J. Lim; Elizabeth E. Gardiner; Woei Ming Lee

QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY

Yujie Zheng^*, Samantha J. Montague, Yean J. Lim, Elizabeth E. Gardiner, Woei Ming Lee

^*Corresponding author for this work

Cardiovascular Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, we use a high resolution multimodality label-free flow imaging assay to investigate the effect of platelet shedding of key adheso-signalling receptors in thrombus formation assay by inhibiting activation-induced metalloproteolytic cleavage of platelet receptors (GPIb of the GPIb-IX-V complex and GPVI).

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
Publisher	Chemical and Biological Microsystems Society
Pages	1533-1534
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

Bibliographical note

Funding Information:
Using COSI, we unpicks intra-thrombus mass and adhesive platelet-only activity events under fluid shear stress. With a broad range metalloproteinase inhibitor blocking shedding of platelet adhesion receptors, we shown the elevated adhesive platelet activity at average of 42.1 µm/s and minimal change in intra-thrombus mass. ACKNOWLEDGEMENTS This work was supported by grants Australian Research Council (DP200100364, DP190100039, DE160100843) and ANU Major Equipment grant (15MEC36, 16MEC26). REFERENCES [1] J.W. Dobrucki, et al. Biophys. J., vol. 93, no.5, pp. 1778-1786, 2007. [2] Y. Zheng, et al. Biophys. J., vol. 120, no. 5, pp.791-804, 2021 CONTACT Yujie Zheng; email: yujie.zheng@anu.eud.au

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

interferometry
label - free
microfluidics
Platelet
receptor shedding
scattering
thrombus

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Access to Document

https://www.proceedings.com/64786.htmlLicence: None: All rights reserved

Cite this

Zheng, Y., Montague, S. J., Lim, Y. J., Gardiner, E. E., & Lee, W. M. (2021). QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) (pp. 1533-1534). Chemical and Biological Microsystems Society. https://www.proceedings.com/64786.html

@inproceedings{bd98308cfdaf4993af3f6547c964228c,

title = "QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY",

abstract = "In this paper, we use a high resolution multimodality label-free flow imaging assay to investigate the effect of platelet shedding of key adheso-signalling receptors in thrombus formation assay by inhibiting activation-induced metalloproteolytic cleavage of platelet receptors (GPIb of the GPIb-IX-V complex and GPVI).",

keywords = "interferometry, label - free, microfluidics, Platelet, receptor shedding, scattering, thrombus",

author = "Yujie Zheng and Montague, {Samantha J.} and Lim, {Yean J.} and Gardiner, {Elizabeth E.} and Lee, {Woei Ming}",

note = "Funding Information: Using COSI, we unpicks intra-thrombus mass and adhesive platelet-only activity events under fluid shear stress. With a broad range metalloproteinase inhibitor blocking shedding of platelet adhesion receptors, we shown the elevated adhesive platelet activity at average of 42.1 µm/s and minimal change in intra-thrombus mass. ACKNOWLEDGEMENTS This work was supported by grants Australian Research Council (DP200100364, DP190100039, DE160100843) and ANU Major Equipment grant (15MEC36, 16MEC26). REFERENCES [1] J.W. Dobrucki, et al. Biophys. J., vol. 93, no.5, pp. 1778-1786, 2007. [2] Y. Zheng, et al. Biophys. J., vol. 120, no. 5, pp.791-804, 2021 CONTACT Yujie Zheng; email: yujie.zheng@anu.eud.au Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

pages = "1533--1534",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)",

publisher = "Chemical and Biological Microsystems Society",

}

Zheng, Y, Montague, SJ, Lim, YJ, Gardiner, EE & Lee, WM 2021, QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) . Chemical and Biological Microsystems Society, pp. 1533-1534, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21. <https://www.proceedings.com/64786.html>

QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY. / Zheng, Yujie; Montague, Samantha J.; Lim, Yean J. et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) . Chemical and Biological Microsystems Society, 2021. p. 1533-1534.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY

AU - Zheng, Yujie

AU - Montague, Samantha J.

AU - Lim, Yean J.

AU - Gardiner, Elizabeth E.

AU - Lee, Woei Ming

N1 - Funding Information: Using COSI, we unpicks intra-thrombus mass and adhesive platelet-only activity events under fluid shear stress. With a broad range metalloproteinase inhibitor blocking shedding of platelet adhesion receptors, we shown the elevated adhesive platelet activity at average of 42.1 µm/s and minimal change in intra-thrombus mass. ACKNOWLEDGEMENTS This work was supported by grants Australian Research Council (DP200100364, DP190100039, DE160100843) and ANU Major Equipment grant (15MEC36, 16MEC26). REFERENCES [1] J.W. Dobrucki, et al. Biophys. J., vol. 93, no.5, pp. 1778-1786, 2007. [2] Y. Zheng, et al. Biophys. J., vol. 120, no. 5, pp.791-804, 2021 CONTACT Yujie Zheng; email: yujie.zheng@anu.eud.au Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021

Y1 - 2021

N2 - In this paper, we use a high resolution multimodality label-free flow imaging assay to investigate the effect of platelet shedding of key adheso-signalling receptors in thrombus formation assay by inhibiting activation-induced metalloproteolytic cleavage of platelet receptors (GPIb of the GPIb-IX-V complex and GPVI).

AB - In this paper, we use a high resolution multimodality label-free flow imaging assay to investigate the effect of platelet shedding of key adheso-signalling receptors in thrombus formation assay by inhibiting activation-induced metalloproteolytic cleavage of platelet receptors (GPIb of the GPIb-IX-V complex and GPVI).

KW - interferometry

KW - label - free

KW - microfluidics

KW - Platelet

KW - receptor shedding

KW - scattering

KW - thrombus

UR - http://www.scopus.com/inward/record.url?scp=85136935655&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85136935655

SP - 1533

EP - 1534

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

QUANTIFICATION OF THROMBUS KINETICS THROUGH INHIBITING RECEPTOR SHEDDING WITH LABEL-FREE IMAGING FLOW ASSAY

Abstract

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this