Recent Advances in Metalloproteomics

James P.C. Coverdale; Sirilata Polepalli; Marco A.Z. Arruda; Ana B. Santos da Silva; Alan J. Stewart; Claudia A. Blindauer

doi:10.3390/biom14010104

Recent Advances in Metalloproteomics

James P.C. Coverdale, Sirilata Polepalli, Marco A.Z. Arruda, Ana B. Santos da Silva, Alan J. Stewart^*, Claudia A. Blindauer^*

^*Corresponding author for this work

Pharmacy

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Abstract

Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

Original language	English
Article number	104
Number of pages	31
Journal	Biomolecules
Volume	14
Issue number	1
DOIs	https://doi.org/10.3390/biom14010104
Publication status	Published - 13 Jan 2024

Bibliographical note

Funding Information:
This work was funded by the BBSRC (Biological and Biotechnological Sciences Research Council), Grant No BB/V014684/1. We also thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), Grant Nos. 2014/50867-3, 2018/25207-0 and 2020/08543-7, and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), Grant No 303231/2020-3. JPCC thanks the Royal Society of Chemistry for an Enablement grant (E22-1637945680). The APC was funded by MDPI.

Publisher Copyright:
© 2024 by the authors.

Keywords

essential metals
ligand exchange kinetics
metallodrugs
metalloproteome
xenobiotic metals

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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Cite this

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title = "Recent Advances in Metalloproteomics",

abstract = "Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.",

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note = "Funding Information: This work was funded by the BBSRC (Biological and Biotechnological Sciences Research Council), Grant No BB/V014684/1. We also thank FAPESP (Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo), Grant Nos. 2014/50867-3, 2018/25207-0 and 2020/08543-7, and CNPq (Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnologico), Grant No 303231/2020-3. JPCC thanks the Royal Society of Chemistry for an Enablement grant (E22-1637945680). The APC was funded by MDPI. Publisher Copyright: {\textcopyright} 2024 by the authors.",

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AU - Stewart, Alan J.

AU - Blindauer, Claudia A.

N1 - Funding Information: This work was funded by the BBSRC (Biological and Biotechnological Sciences Research Council), Grant No BB/V014684/1. We also thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), Grant Nos. 2014/50867-3, 2018/25207-0 and 2020/08543-7, and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), Grant No 303231/2020-3. JPCC thanks the Royal Society of Chemistry for an Enablement grant (E22-1637945680). The APC was funded by MDPI. Publisher Copyright: © 2024 by the authors.

PY - 2024/1/13

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N2 - Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

AB - Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

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Recent Advances in Metalloproteomics

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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