TY - JOUR
T1 - Prevalence of human papilloma virus (HPV) genotypes between outpatients males and females referred to seven laboratories in Tehran, Iran
AU - Rezaee Azhar, Iman
AU - Yaghoobi, Mahmood
AU - Mossalaeie, Mir Majid
AU - Kollaee Darabi, Abolghasem
AU - Nejadeh, Amir Houshang
AU - Jamshidi, Mahbobeh
AU - Ahani, Ali
AU - Karkhane Mahmoodi, Masoud
AU - Ghalichi, Leila
AU - Shabanzadeh, Ayda
AU - Ataei-Pirkooh, Angila
AU - Marjani, Arezoo
AU - Khamseh, Azam
AU - Shafiei, Motahareh
AU - Hosseini, Parastoo
AU - Soltani, Saber
AU - Zandi, Milad
AU - Ghafari, Parsa
AU - Aboofazeli, Amir
AU - Ghaziasadi, Azam
AU - Jazayeri, Seyed Mohammad
N1 - Funding Information:
The authors acknowledge the help of members of Aramesh, Nejadeh, Parseh, Mandel, Laleh hospital and Noor medical laboratories.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Background: Human papilloma virus (HPV) causes the most common sexually-transmitted infection especially among sexually-active individuals. The aim of study was to characterize the molecular characterization of HPV genotypes between 5176 female and male patients. Methods: HPV DNA was extracted from genital swabs of the study participants and amplified by Real Time Polymerase Chain Reaction (PCR). Genotyping was performed for 2525 cases using REALQUALITY RQ-Multi HPV Detection Kit for the identification of 14 high risk (HR) and 2 low risk (LR) HPV genotypes. Demographic figures were analyzed in correlation with virological data statistically. Results: Out of 5176 cases from 7 laboratories, 2727 (53%) were positive for HPV, of which. 2372(87%) women and 355 (13%) men were HPV positive. However, in an intra-gender analysis, positive rate was higher in men (355/637, 55.7%) than in women (2372/4539, 52%; P value 0.007). HPV positive patients were younger than negative individuals. Positive rate was higher among age categories 20–40. Genotyping was performed for 2525 cases. Out of 1219 (48%) patients who contained single genotypes, 566 (22%) and 653 (26%) harboured HR and LR genotypes, respectively. In females and males, 1189 (54%) and 117 (37%) contained multiple genotypes. No substantial associations were found between different age categories and HR/LR and multiple genotypes distribution. Conclusion: The prevalence of HPV infection in both genders was high. However, men had a higher rate of infection. These observations highlighted the necessity for a plan for targeted education to younger population in the society as well as application of infection control measures against HPV infection, especially in terms of general population mass HPV vaccination.
AB - Background: Human papilloma virus (HPV) causes the most common sexually-transmitted infection especially among sexually-active individuals. The aim of study was to characterize the molecular characterization of HPV genotypes between 5176 female and male patients. Methods: HPV DNA was extracted from genital swabs of the study participants and amplified by Real Time Polymerase Chain Reaction (PCR). Genotyping was performed for 2525 cases using REALQUALITY RQ-Multi HPV Detection Kit for the identification of 14 high risk (HR) and 2 low risk (LR) HPV genotypes. Demographic figures were analyzed in correlation with virological data statistically. Results: Out of 5176 cases from 7 laboratories, 2727 (53%) were positive for HPV, of which. 2372(87%) women and 355 (13%) men were HPV positive. However, in an intra-gender analysis, positive rate was higher in men (355/637, 55.7%) than in women (2372/4539, 52%; P value 0.007). HPV positive patients were younger than negative individuals. Positive rate was higher among age categories 20–40. Genotyping was performed for 2525 cases. Out of 1219 (48%) patients who contained single genotypes, 566 (22%) and 653 (26%) harboured HR and LR genotypes, respectively. In females and males, 1189 (54%) and 117 (37%) contained multiple genotypes. No substantial associations were found between different age categories and HR/LR and multiple genotypes distribution. Conclusion: The prevalence of HPV infection in both genders was high. However, men had a higher rate of infection. These observations highlighted the necessity for a plan for targeted education to younger population in the society as well as application of infection control measures against HPV infection, especially in terms of general population mass HPV vaccination.
KW - Cervical cancer
KW - Human papilloma virus
KW - Sexually-transmitted infections
UR - http://www.scopus.com/inward/record.url?scp=85126143642&partnerID=8YFLogxK
U2 - 10.1186/s13027-022-00421-7
DO - 10.1186/s13027-022-00421-7
M3 - Article
AN - SCOPUS:85126143642
SN - 1750-9378
VL - 17
JO - Infectious Agents and Cancer
JF - Infectious Agents and Cancer
IS - 1
M1 - 7
ER -
