Flow cytometric analysis of CD4+ T cell re-activation following anti-PD1 immunotherapy in a transgenic mouse model

Lozan Sheriff; David Bending

doi:10.1016/j.xpro.2022.101161

Flow cytometric analysis of CD4+ T cell re-activation following anti-PD1 immunotherapy in a transgenic mouse model

Lozan Sheriff, David Bending^*

^*Corresponding author for this work

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

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Abstract

This protocol uses the Tg4 Nr4a3-Tocky mouse model to recalibrate T cell activation thresholds and reveals the role that immune checkpoints play in controlling T cell activation. The example approach here uses flow cytometry to characterize quantitative and qualitative changes in splenic CD4⁺ T cells reactivated in the presence of anti-PD1 immunotherapy. The protocol is optimized for studying anti-PD1 pathway blockade only. The protocol is not compatible with cellular fixation, and T cells should be analyzed immediately after staining.

For complete details on the use and execution of this protocol, please refer to Elliot et al. (2021).

Original language	English
Article number	101161
Number of pages	11
Journal	STAR Protocols
Volume	3
Issue number	1
Early online date	3 Feb 2022
DOIs	https://doi.org/10.1016/j.xpro.2022.101161
Publication status	Published - 18 Mar 2022

Bibliographical note

Funding Information:
This work is funded by the MRC ( MR/V009052/1 ). The graphical abstract and Figure 1 were created using https://biorender.com .

Publisher Copyright:
© 2022 The Author(s)

Keywords

Cancer
Cell Biology
Flow Cytometry/Mass Cytometry
Immunology
Model Organisms

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)
Medicine(all)

Access to Document

10.1016/j.xpro.2022.101161Licence: Creative Commons: Attribution (CC BY)

SheriffL2022FlowFinal published version, 2.31 MBLicence: Creative Commons: Attribution (CC BY)

Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers
Bending, D.
Medical Research Council
1/02/21 → 31/01/26
Project: Research Councils

Cite this

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abstract = "This protocol uses the Tg4 Nr4a3-Tocky mouse model to recalibrate T cell activation thresholds and reveals the role that immune checkpoints play in controlling T cell activation. The example approach here uses flow cytometry to characterize quantitative and qualitative changes in splenic CD4+ T cells reactivated in the presence of anti-PD1 immunotherapy. The protocol is optimized for studying anti-PD1 pathway blockade only. The protocol is not compatible with cellular fixation, and T cells should be analyzed immediately after staining.For complete details on the use and execution of this protocol, please refer to Elliot et al. (2021).",

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KW - Model Organisms

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Flow cytometric analysis of CD4+ T cell re-activation following anti-PD1 immunotherapy in a transgenic mouse model

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Projects

Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers

Cite this