Abstract
Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of cis-regulatory elements in vivo by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as gata2a, notch1b and dll4. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the cis-regulatory sequences that shape the zebrafish vascular network.
| Original language | English |
|---|---|
| Article number | 891538 |
| Pages (from-to) | 891538 |
| Number of pages | 16 |
| Journal | Frontiers in cell and developmental biology |
| Volume | 10 |
| DOIs | |
| Publication status | Published - 9 May 2022 |
Bibliographical note
Funding Information:KK was supported by the Wallenberg Academy Fellowship (2017.0144), Ragnar Söderbergs Fellowship (M13/17), Vetenskapsådet (VR-MH-2016-01437) and Jeanssons Foundation. RM was supported by a Birmingham Fellowship (University of Birmingham, United Kingdom).
Publisher Copyright:
Copyright © 2022 Panara, Monteiro and Koltowska.
Keywords
- ATAC-seq
- blood endothelial cells
- ChIP-seq
- chromatin structure
- conserved non-coding elements
- enhancers
- epigenetics
- lymphatic endothelial cells
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology