Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Jake G. Carter; Lorea Orueta Iturbe; Jean-Louis H. A. Duprey; Ian R. Carter; Craig D. Southern; Marium Rana; Andrew Bosworth; Andrew D. Beggs; Matthew R. Hicks; James H. R. Tucker; Timothy R. Dafforn

doi:10.1101/2020.12.31.20248236

Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Jake G. Carter, Lorea Orueta Iturbe, Jean-Louis H. A. Duprey, Ian R. Carter, Craig D. Southern, Marium Rana, Andrew Bosworth, Andrew D. Beggs, Matthew R. Hicks, James H. R. Tucker, Timothy R. Dafforn

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Abstract

We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	medRxiv
Volume	2021
DOIs	https://doi.org/10.1101/2020.12.31.20248236
Publication status	Published - 4 Jan 2021

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2020.12.31.20248236v1.fullSubmitted manuscript, 467 KBLicence: Creative Commons: Attribution (CC BY)

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title = "Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR",

abstract = "We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.",

author = "Carter, {Jake G.} and Iturbe, {Lorea Orueta} and Duprey, {Jean-Louis H. A.} and Carter, {Ian R.} and Southern, {Craig D.} and Marium Rana and Andrew Bosworth and Beggs, {Andrew D.} and Hicks, {Matthew R.} and Tucker, {James H. R.} and Dafforn, {Timothy R.}",

year = "2021",

month = jan,

day = "4",

doi = "10.1101/2020.12.31.20248236",

language = "English",

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T1 - Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

AU - Carter, Jake G.

AU - Iturbe, Lorea Orueta

AU - Duprey, Jean-Louis H. A.

AU - Carter, Ian R.

AU - Southern, Craig D.

AU - Rana, Marium

AU - Bosworth, Andrew

AU - Beggs, Andrew D.

AU - Hicks, Matthew R.

AU - Tucker, James H. R.

AU - Dafforn, Timothy R.

PY - 2021/1/4

Y1 - 2021/1/4

N2 - We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.

AB - We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.

U2 - 10.1101/2020.12.31.20248236

DO - 10.1101/2020.12.31.20248236

M3 - Article

VL - 2021

SP - 1

EP - 7

JO - medRxiv

JF - medRxiv

ER -

Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Abstract

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