Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia

Michaela Gregorova; Daniel Morse; Tarcisio Brignoli; Joseph Steventon; Fergus Hamilton; Mahableshwar Albur; David Arnold; Matthew Thomas; Alice Halliday; Holly Baum; Christopher Rice; Matthew B. Avison; Andrew D. Davidson; Marianna Santopaolo; Elizabeth Oliver; Anu Goenka; Adam Finn; Linda Wooldridge; Borko Amulic; Rosemary J. Boyton; Daniel M. Altmann; David K. Butler; Claire McMurray; Joanna Stockton; Sam Nicholls; Charles Cooper; Nicholas Loman; Michael J. Cox; Laura Rivino; Ruth C. Massey

doi:10.7554/eLife.63430

Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia

Michaela Gregorova, Daniel Morse, Tarcisio Brignoli, Joseph Steventon, Fergus Hamilton, Mahableshwar Albur, David Arnold, Matthew Thomas, Alice Halliday, Holly Baum, Christopher Rice, Matthew B. Avison, Andrew D. Davidson, Marianna Santopaolo, Elizabeth Oliver, Anu Goenka, Adam Finn, Linda Wooldridge, Borko Amulic, Rosemary J. BoytonDaniel M. Altmann, David K. Butler, Claire McMurray, Joanna Stockton, Sam Nicholls, Charles Cooper, Nicholas Loman, Michael J. Cox, Laura Rivino^*, Ruth C. Massey

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we describe the case of a COVID-19 patient who developed recurring ventilator- associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed massive and escalating levels of T-cell activation. These were both SARS-CoV-2 and P. aeruginosa specific, and bystander activated, which may have contributed to this patient’s persistent symptoms and radiological changes.

Original language	English
Article number	e63430
Pages (from-to)	1-13
Number of pages	13
Journal	eLife
Volume	9
DOIs	https://doi.org/10.7554/eLife.63430
Publication status	Published - Dec 2020

Bibliographical note

Funding Information:
We would like to thank Kapil Gupta and Imre Berger for kindly providing us the spike protein, Natalie Di Bartolo and Ashley Toye for kindly providing us the N protein, both used for the SARS-CoV-2 serology work. The authors wish to acknowledge the assistance of Dr. Andrew Herman and Helen Rice and the University of Bristol Faculty of Biomedical Sciences Flow Cytometry Facility. We would also like to thank Keith Jolley and the Bristol University UNCOVER team for helpful discussions during the execution of this work and preparation of the manuscript. This work was supported by donations to Southmead Hospital Charity (Registered Charity Number: 1055900), by the Wellcome Trust (reference number: 212258/Z/18/Z) and by the Elizabeth Blackwell Institute, University of Bristol, with funding from the University’s alumni and friends. DKB is supported by a Cystic Fibrosis Trust PhD studentship (CF Trust SRC 015). RJB and DMA are supported by UKRI (MR/S019553/1 and MR/ R02622X/1)

Publisher Copyright:
© Gregorova et al.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

Neuroscience(all)
Immunology and Microbiology(all)
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.7554/eLife.63430

1 Preprint

Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia.
Cox, M., 22 Sept 2020.
Research output: Working paper/Preprint › Preprint

Cite this

Gregorova, M., Morse, D., Brignoli, T., Steventon, J., Hamilton, F., Albur, M., Arnold, D., Thomas, M., Halliday, A., Baum, H., Rice, C., Avison, M. B., Davidson, A. D., Santopaolo, M., Oliver, E., Goenka, A., Finn, A., Wooldridge, L., Amulic, B., ... Massey, R. C. (2020). Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia. eLife, 9, 1-13. Article e63430. https://doi.org/10.7554/eLife.63430

@article{54ef001720454a34b89a80f8bf8734c3,

title = "Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia",

abstract = "Here, we describe the case of a COVID-19 patient who developed recurring ventilator- associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed massive and escalating levels of T-cell activation. These were both SARS-CoV-2 and P. aeruginosa specific, and bystander activated, which may have contributed to this patient{\textquoteright}s persistent symptoms and radiological changes.",

author = "Michaela Gregorova and Daniel Morse and Tarcisio Brignoli and Joseph Steventon and Fergus Hamilton and Mahableshwar Albur and David Arnold and Matthew Thomas and Alice Halliday and Holly Baum and Christopher Rice and Avison, {Matthew B.} and Davidson, {Andrew D.} and Marianna Santopaolo and Elizabeth Oliver and Anu Goenka and Adam Finn and Linda Wooldridge and Borko Amulic and Boyton, {Rosemary J.} and Altmann, {Daniel M.} and Butler, {David K.} and Claire McMurray and Joanna Stockton and Sam Nicholls and Charles Cooper and Nicholas Loman and Cox, {Michael J.} and Laura Rivino and Massey, {Ruth C.}",

note = "Funding Information: We would like to thank Kapil Gupta and Imre Berger for kindly providing us the spike protein, Natalie Di Bartolo and Ashley Toye for kindly providing us the N protein, both used for the SARS-CoV-2 serology work. The authors wish to acknowledge the assistance of Dr. Andrew Herman and Helen Rice and the University of Bristol Faculty of Biomedical Sciences Flow Cytometry Facility. We would also like to thank Keith Jolley and the Bristol University UNCOVER team for helpful discussions during the execution of this work and preparation of the manuscript. This work was supported by donations to Southmead Hospital Charity (Registered Charity Number: 1055900), by the Wellcome Trust (reference number: 212258/Z/18/Z) and by the Elizabeth Blackwell Institute, University of Bristol, with funding from the University{\textquoteright}s alumni and friends. DKB is supported by a Cystic Fibrosis Trust PhD studentship (CF Trust SRC 015). RJB and DMA are supported by UKRI (MR/S019553/1 and MR/ R02622X/1) Publisher Copyright: {\textcopyright} Gregorova et al. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2020",

month = dec,

doi = "10.7554/eLife.63430",

language = "English",

volume = "9",

pages = "1--13",

journal = "eLife",

issn = "2050-084X",

publisher = "eLife Sciences Publications",

}

Gregorova, M, Morse, D, Brignoli, T, Steventon, J, Hamilton, F, Albur, M, Arnold, D, Thomas, M, Halliday, A, Baum, H, Rice, C, Avison, MB, Davidson, AD, Santopaolo, M, Oliver, E, Goenka, A, Finn, A, Wooldridge, L, Amulic, B, Boyton, RJ, Altmann, DM, Butler, DK, McMurray, C, Stockton, J, Nicholls, S, Cooper, C, Loman, N , Cox, MJ, Rivino, L & Massey, RC 2020, 'Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia', eLife, vol. 9, e63430, pp. 1-13. https://doi.org/10.7554/eLife.63430

TY - JOUR

T1 - Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia

AU - Gregorova, Michaela

AU - Morse, Daniel

AU - Brignoli, Tarcisio

AU - Steventon, Joseph

AU - Hamilton, Fergus

AU - Albur, Mahableshwar

AU - Arnold, David

AU - Thomas, Matthew

AU - Halliday, Alice

AU - Baum, Holly

AU - Rice, Christopher

AU - Avison, Matthew B.

AU - Davidson, Andrew D.

AU - Santopaolo, Marianna

AU - Oliver, Elizabeth

AU - Goenka, Anu

AU - Finn, Adam

AU - Wooldridge, Linda

AU - Amulic, Borko

AU - Boyton, Rosemary J.

AU - Altmann, Daniel M.

AU - Butler, David K.

AU - McMurray, Claire

AU - Stockton, Joanna

AU - Nicholls, Sam

AU - Cooper, Charles

AU - Loman, Nicholas

AU - Cox, Michael J.

AU - Rivino, Laura

AU - Massey, Ruth C.

N1 - Funding Information: We would like to thank Kapil Gupta and Imre Berger for kindly providing us the spike protein, Natalie Di Bartolo and Ashley Toye for kindly providing us the N protein, both used for the SARS-CoV-2 serology work. The authors wish to acknowledge the assistance of Dr. Andrew Herman and Helen Rice and the University of Bristol Faculty of Biomedical Sciences Flow Cytometry Facility. We would also like to thank Keith Jolley and the Bristol University UNCOVER team for helpful discussions during the execution of this work and preparation of the manuscript. This work was supported by donations to Southmead Hospital Charity (Registered Charity Number: 1055900), by the Wellcome Trust (reference number: 212258/Z/18/Z) and by the Elizabeth Blackwell Institute, University of Bristol, with funding from the University’s alumni and friends. DKB is supported by a Cystic Fibrosis Trust PhD studentship (CF Trust SRC 015). RJB and DMA are supported by UKRI (MR/S019553/1 and MR/ R02622X/1) Publisher Copyright: © Gregorova et al. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2020/12

Y1 - 2020/12

N2 - Here, we describe the case of a COVID-19 patient who developed recurring ventilator- associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed massive and escalating levels of T-cell activation. These were both SARS-CoV-2 and P. aeruginosa specific, and bystander activated, which may have contributed to this patient’s persistent symptoms and radiological changes.

AB - Here, we describe the case of a COVID-19 patient who developed recurring ventilator- associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed massive and escalating levels of T-cell activation. These were both SARS-CoV-2 and P. aeruginosa specific, and bystander activated, which may have contributed to this patient’s persistent symptoms and radiological changes.

UR - http://www.scopus.com/inward/record.url?scp=85099320452&partnerID=8YFLogxK

U2 - 10.7554/eLife.63430

DO - 10.7554/eLife.63430

M3 - Article

C2 - 33331820

AN - SCOPUS:85099320452

SN - 2050-084X

VL - 9

SP - 1

EP - 13

JO - eLife

JF - eLife

M1 - e63430

ER -

Post-acute COVID-19 associated with evidence of bystander t-cell activation and a recurring antibiotic-resistant bacterial pneumonia

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Fingerprint

Research output

Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia.

Cite this