Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Yuan Zhao; Mohamed  Uduman; Jacqueline HY Siu; Thomas Tull; Jeremy D Sanderson; Yu-Chang Bryan Wu; Julian Q Yhou; Nedyalko Petrov; Katrina Todd; Konstantia-Maria Chavele; William Guesdon; Wayel Jassem; Anna Vossenkamper; David P D’Cruz; David J  Fear; Susan  John; Dagmar Scheel-Toellner; Claire  Hopkins; Estefania  Moreno; Natalie L Woodman; Francesca  Ciccarelli; Susanne  Heck; Steven H  Kleinstein; Mats Bemark; Jo Spencer; Richard J Ellis

doi:10.1038/s41467-018-06089-1

Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Yuan Zhao, Mohamed Uduman, Jacqueline HY Siu, Thomas Tull, Jeremy D Sanderson, Yu-Chang Bryan Wu, Julian Q Yhou, Nedyalko Petrov, Katrina Todd, Konstantia-Maria Chavele, William Guesdon, Wayel Jassem, Anna Vossenkamper, David P D’Cruz, David J Fear, Susan John, Dagmar Scheel-Toellner, Claire Hopkins, Estefania Moreno, Natalie L WoodmanFrancesca Ciccarelli, Susanne Heck, Steven H Kleinstein, Mats Bemark, Jo Spencer, Richard J Ellis

Inflammation and Ageing

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Abstract

Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired

Original language	English
Article number	3857
Journal	Nature Communications
DOIs	https://doi.org/10.1038/s41467-018-06089-1
Publication status	Published - 21 Sept 2018

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Zhao, Y., Uduman, M., Siu, J. HY., Tull, T., Sanderson, J. D., Wu, Y-C. B., Yhou, J. Q., Petrov, N., Todd, K., Chavele, K-M., Guesdon, W., Jassem, W., Vossenkamper, A., D’Cruz, D. P., Fear, D. J., John, S., Scheel-Toellner, D., Hopkins, C., Moreno, E., ... Ellis, R. J. (2018). Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue. Nature Communications, Article 3857. https://doi.org/10.1038/s41467-018-06089-1

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title = "Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue",

abstract = "Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature na{\"i}ve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired",

author = "Yuan Zhao and Mohamed Uduman and Siu, {Jacqueline HY} and Thomas Tull and Sanderson, {Jeremy D} and Wu, {Yu-Chang Bryan} and Yhou, {Julian Q} and Nedyalko Petrov and Katrina Todd and Konstantia-Maria Chavele and William Guesdon and Wayel Jassem and Anna Vossenkamper and D{\textquoteright}Cruz, {David P} and Fear, {David J} and Susan John and Dagmar Scheel-Toellner and Claire Hopkins and Estefania Moreno and Woodman, {Natalie L} and Francesca Ciccarelli and Susanne Heck and Kleinstein, {Steven H} and Mats Bemark and Jo Spencer and Ellis, {Richard J}",

year = "2018",

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Zhao, Y, Uduman, M, Siu, JHY, Tull, T, Sanderson, JD, Wu, Y-CB, Yhou, JQ, Petrov, N, Todd, K, Chavele, K-M, Guesdon, W, Jassem, W, Vossenkamper, A, D’Cruz, DP, Fear, DJ, John, S, Scheel-Toellner, D, Hopkins, C, Moreno, E, Woodman, NL, Ciccarelli, F, Heck, S, Kleinstein, SH, Bemark, M, Spencer, J & Ellis, RJ 2018, 'Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue', Nature Communications. https://doi.org/10.1038/s41467-018-06089-1

TY - JOUR

T1 - Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

AU - Zhao, Yuan

AU - Uduman, Mohamed

AU - Siu, Jacqueline HY

AU - Tull, Thomas

AU - Sanderson, Jeremy D

AU - Wu, Yu-Chang Bryan

AU - Yhou, Julian Q

AU - Petrov, Nedyalko

AU - Todd, Katrina

AU - Chavele, Konstantia-Maria

AU - Guesdon, William

AU - Jassem, Wayel

AU - Vossenkamper, Anna

AU - D’Cruz, David P

AU - Fear, David J

AU - John, Susan

AU - Scheel-Toellner, Dagmar

AU - Hopkins, Claire

AU - Moreno, Estefania

AU - Woodman, Natalie L

AU - Ciccarelli, Francesca

AU - Heck, Susanne

AU - Kleinstein, Steven H

AU - Bemark, Mats

AU - Spencer, Jo

AU - Ellis, Richard J

PY - 2018/9/21

Y1 - 2018/9/21

N2 - Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired

AB - Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired

U2 - 10.1038/s41467-018-06089-1

DO - 10.1038/s41467-018-06089-1

M3 - Article

SN - 2041-1723

JO - Nature Communications

JF - Nature Communications

M1 - 3857

ER -

Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Abstract

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