Abstract
Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.
Original language | English |
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Pages (from-to) | 1961-71 |
Number of pages | 11 |
Journal | Journal of Biomedical Materials Research Part A |
Volume | 102 |
Issue number | 6 |
Early online date | 12 Jul 2013 |
DOIs | |
Publication status | Published - 1 Jun 2014 |
Keywords
- Cell Line
- Cell Survival
- Coated Materials, Biocompatible
- Dental Implants
- Humans
- Lupinus
- Malus
- Osteoblasts
- Pectins
- Journal Article
- Research Support, Non-U.S. Gov't
- dental implant
- polysaccharide
- surface modification
- SaOS‐2 cell line
- Osseointegration